Efficacy of real-time polymerase chain reaction for rapid diagnosis of endobronchial tuberculosis

Polymerase Chain Reaction for the Diagnosis of Tuberculosis

Dear Editor-in-Chief We read with interest the study by Khazaei et al. (1) in which the authors have nicely concluded that PCR is more sensitive test than Ziehl-Neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (TB). They have rightly pointed to use PCR, selectively, in acidfast bacilli negative paucibacillary forms of TB. However, we intend to highlight few...

Use of polymerase chain reaction for rapid diagnosis of tuberculosis.

A DNA amplification assay using the polymerase chain reaction technique designed for the rapid identification of Mycobacterium bovis organisms was used to test 211 human mycobacterial isolates and 177 clinical specimens previously submitted for routine mycobacterial culture. The procedures described could be used by routine or specialist laboratories for identification of M. tuberculosis comple...

Rapid Diagnosis of Genital Tuberculosis by Real-time Polymerase Chain Reaction

Objective: Rapid diagnosis of genital tuberculosis (GTB) is essential as it is an important cause of infertility among women. Diagnosis can be done by imaging techniques, direct visualization by endoscopy, serology, histopathology, culture and polymerase chain reaction (PCR) tests. Laparoscopy detects macroscopic changes only; histology is only suggestive and not confirmatory unless acid fast b...

polymerase chain reaction for the diagnosis of tuberculosis

dear editor-in-chief we read with interest the study by khazaei et al. (1) in which the authors have nicely concluded that pcr is more sensitive test than ziehl-neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (tb). they have rightly pointed to use pcr, selectively, in acidfast bacilli negative paucibacillary forms of tb. however, we intend to highlight few ...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*